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Reliability of Angiotensin-Converting Enzyme Gene Polymorphism in Testing
- Bernhard R. Winkelmann, MD;
- Markus Nauck, MD; and
- Winfried Marz, MD
- Herzzentrum Ludwigshafen, 67063 Ludwigshafen, Germany Klinikum der Albert Ludwigs-Universitat, 79106 Freiburg, Germany
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IN RESPONSE:
Odawara and Yamashita question the reliability of the methods we used for genotyping the insertion/deletion polymorphism of the ACE gene [1, 2]. It has been emphasized that using primers that flank the polymorphic region may result in overestimation of the DD genotype because of preferential amplification of the D allele [2, 3]. We therefore reassessed all our samples using the method proposed by Shanmugan and colleagues [2], as described in the Methods section of our report. We also used the method of Lindpaintner and associates [3] to retype the samples that were previously found to be DD homozygous; this method also uses an insertion-specific primer. This reassessment completely confirmed the data reported in our study. Therefore, we are confident that mistyping did not jeopardize the validity of our data.
Bernhard R. Winkelmann, MD
Herzzentrum Ludwigshafen; 67063 Ludwigshafen, Germany
Markus Nauck, MD
Winfried Marz, MD
Klinikum der Albert Ludwigs-Universitat; 79106 Freiburg, Germany
The Editors welcome submissions for possible publication in the Letters section. Authors of letters should:
•Include no more than 300 words of text, three authors, and five references
•Type with double-spacing
•Send three copies of the letter, an authors' form signed by all authors, and a cover letter describing any conflicts of interest related to the contents of the letter.
Letters commenting on an Annals article will be considered if they are received within 6 weeks of the time the article was published. Only some of the letters received can be published. Published letters are edited and may be shortened; tables and figures are included only selectively. Authors will be notified that the letter has been received. If the letter is selected for publication, the author will be notified about 3 weeks before the publication date. Unpublished letters cannot be returned.
Annals welcomes electronically submitted letters.
- Copyright ©2004 by the American College of Physicians
