Figure 3. The 21-hydroxylase-deficient mouse.Top. Steroidogenic acute regulatory protein (StAR) expression is increased in the adrenal glands of mice with 21-hydroxylase deficiency, as compared with wild-type animals. Quantitative steroidogenic acute regulatory protein was determined by TaqMan PCR Core Reagents Kit (Applied Biosystems, Foster City, California) (n = 4 adrenal glands; P < 0.05). Amounts of RNA were calculated with relative standard curves for both steroidogenic acute regulatory protein and 18S. The amount of messenger RNA was corrected by division by the amount of 18S RNA in each sample. Middle. Electroµgraph showing catecholamine-storing secretory vesicles in chromaffin cells of control animals (size range, 50 to 450 nm). Bottom. Electroµgraph of chro-maffin cells. Secretory granules are markedly reduced in chromaffin cells of 21-hydroxylase-deficient mice. The remaining granules are predominantly electron-dense, norepinephrine-containing vesicles, lying in large lucent vacuoles (arrows). For parts B and C, stain is uranyl acetate and lead citrate, and magnification is x 15 000. MIT = mitochondria.

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