REPLY
Reliability of Angiotensin-Converting Enzyme Gene Polymorphism in Testing
Bernhard R. Winkelmann, MD;
Markus Nauck, MD; and
Winfried Marz, MD
15 May 1997 | Volume 126 Issue 10 | Page 828
IN RESPONSE:
Odawara and Yamashita question the reliability of the methods we used for genotyping the insertion/deletion polymorphism of the ACE gene [1, 2]. It has been emphasized that using primers that flank the polymorphic region may result in overestimation of the DD genotype because of preferential amplification of the D allele [2, 3]. We therefore reassessed all our samples using the method proposed by Shanmugan and colleagues [2], as described in the Methods section of our report. We also used the method of Lindpaintner and associates [3] to retype the samples that were previously found to be DD homozygous; this method also uses an insertion-specific primer. This reassessment completely confirmed the data reported in our study. Therefore, we are confident that mistyping did not jeopardize the validity of our data.
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Author and Article Information
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Herzzentrum Ludwigshafen, 67063 Ludwigshafen, Germany
Klinikum der Albert Ludwigs-Universitat, 79106 Freiburg, Germany
1. Rigat B, Hubert C, Corvol P, Soubrier F. PCR detection of the insertion/deletion polymorphism of the human angiotensin converting enzyme gene (DCP1) (dipeptidyl carboxypeptidase 1). Nucleic Acids Res. 1992; 20:1433.
2. Shanmugan V, Sell KW, Saha BK. Mistyping ACE heterozygotes. PCR Methods Appl. 1993; 3:120-1.
3. Lindpaintner K, Pfeffer MA, Kreutz R, Stampfer MJ, Grodstein F, LaMotte F, et al. A prospective evaluation of an angiotensin-converting-enzyme gene polymorphism and the risk of ischemic heart disease. N Engl J Med. 1995; 332:706-11.
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